ABCG2 p.Asn557Ala
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PMID: 15875186
[PubMed]
Mohrmann K et al: "Absence of N-linked glycosylation does not affect plasma membrane localization of breast cancer resistance protein (BCRP/ABCG2)."
No.
Sentence
Comment
8
However, BCRP-N557A and the triple mutant were mainly localized intracellularly, probably in the endoplasmic reticulum, suggesting that this mutation disrupted proper routing of BCRP.
X
ABCG2 p.Asn557Ala 15875186:8:14
status: VERIFIED45 BCRP-N418A, BCRP-N557A and BCRP-N596A were made using pcDNA3/BCRP wild type (GenBank accession number AY017168; generously provided by Susan Bates) as template.
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ABCG2 p.Asn557Ala 15875186:45:17
status: VERIFIED46 BCRP-N418A/ N557A was made using overlap extension PCR with pGEM-T Easy/BCRP-N418A as template and BCRP-N418A/N557A/N596A was made with pGEM-T Easy/ BCRP-N418A/N557A as template again using overlap extension PCR.
X
ABCG2 p.Asn557Ala 15875186:46:12
status: VERIFIEDX
ABCG2 p.Asn557Ala 15875186:46:110
status: VERIFIEDX
ABCG2 p.Asn557Ala 15875186:46:160
status: VERIFIED106 In BCRP-N557A transfectants, only the lower band was detected. As this band was PNGase F sensitive, BCRP-N557A was still glycosylated.
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ABCG2 p.Asn557Ala 15875186:106:8
status: VERIFIEDX
ABCG2 p.Asn557Ala 15875186:106:105
status: VERIFIED130 These results suggest that both BCRP-N557A and the triple mutant were not properly targeted to the plasma membrane as a consequence of the N557A mutation.
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ABCG2 p.Asn557Ala 15875186:130:37
status: VERIFIEDX
ABCG2 p.Asn557Ala 15875186:130:139
status: VERIFIED156 Empty vector pcDNA3 or pcDNA3 with BCRP or mutant BCRP, BCRP-N418A, BCRP-N557A and BCRP-N596A, was transiently transfected into CHO9 cells or MDCKII cells using FuGENE6.
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ABCG2 p.Asn557Ala 15875186:156:73
status: VERIFIED159 N non-glycosylated C core-glycosylated G fully glycosylated In our experiments, BCRP-N557A was apparently core-glycosylated and localized in the ER.
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ABCG2 p.Asn557Ala 15875186:159:87
status: VERIFIED169 We cannot exclude that in our study also a small fraction of BCRP-N557A reaches the plasma membrane.
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ABCG2 p.Asn557Ala 15875186:169:66
status: VERIFIED187 BCRP-N557A and a triple mutant is localized intracellularly.
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ABCG2 p.Asn557Ala 15875186:187:5
status: VERIFIED188 Empty vector pcDNA3 or pcDNA3 with BCRP or mutant BCRP, BCRP-N418A, BCRP-N557A and BCRP-N596A, was transiently transfected into CHO9 cells or MDCKII cells using FuGENE6.
X
ABCG2 p.Asn557Ala 15875186:188:73
status: VERIFIED
PMID: 19200005
[PubMed]
Porcelli L et al: "Intracellular trafficking of MDR transporters and relevance of SNPs."
No.
Sentence
Comment
54
Diop & Hrycyna [30] indicated that glycosylation is not required for transporter function or localization of ABCG2 in the plasma membrane, conversely, Mohrmann suggested that the intracellular localization of BCRP-N557A could disrupt proper routing of the transporter [28].
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ABCG2 p.Asn557Ala 19200005:54:214
status: VERIFIED