ABCG2 p.Arg482Trp
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PMID: 14566825
[PubMed]
Miwa M et al: "Single amino acid substitutions in the transmembrane domains of breast cancer resistance protein (BCRP) alter cross resistance patterns in transfectants."
No.
Sentence
Comment
42
Because FACS analysis showed that certain populations of the methotrexate-resistant PA/R482A and PA/R482W cells did not express BCRP (data not shown), all the R482-mutant BCRP transfectants (except R482K-BCRP transfectant) were further selected with 1 ng/ml of mitoxantrone for 5 days to eliminate untransfected cells.
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ABCG2 p.Arg482Trp 14566825:42:100
status: VERIFIED64 PA/R482N, PA/R482C, PA/R482M, PA/R482S, PA/R482T, PA/R482V, PA/R482A, PA/R482G, PA/R482E PA/R482W and PA/R482D (Group 2) showed higher degrees of resistance to mitoxantrone than to SN-38.
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ABCG2 p.Arg482Trp 14566825:64:92
status: VERIFIED148 As described above, human drug-resistant cell lines MCF-7 AdVp3000, S1-M1-80, MT-4/DOX500 and a mouse drug-resistant line 88.6/D800-B overexpressed R482T- (ACG), R482G- (GGG), R482M- (ATG) and R482S- (AGT) BCRP, respectively.5,6,13,14,23 The other possible mutations are R482W (TGG) and R482K (AAG).
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ABCG2 p.Arg482Trp 14566825:148:271
status: VERIFIED149 PA317 cells expressing R482W- BCRP (PA/R482W) showed somewhat higher levels of resistance to mitoxantrone and doxorubicin than PA/WT2, but the resistance levels were lower than those in PA/ R482G, PA/R482M, PA/R482T and PA/R482S.
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ABCG2 p.Arg482Trp 14566825:149:23
status: VERIFIEDX
ABCG2 p.Arg482Trp 14566825:149:39
status: VERIFIED151 The low-level drug resistance of the transfectants would explain why R482W- and R482K-BCRP were not expressed in drug-selected cell lines.
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ABCG2 p.Arg482Trp 14566825:151:69
status: VERIFIED163 Group 2 members (PA/R482N, PA/R482C, PA/R482M, PA/R482S, PA/R482T, PA/R482V, PA/ R482A, PA/R482G, PA/R482E PA/R482W and PA/R482D) showed higher degrees of resistance to mitoxantrone than to SN-38.
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ABCG2 p.Arg482Trp 14566825:163:110
status: VERIFIED