ABCC7 p.Tyr577Glu
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PMID: 26149808
[PubMed]
Chong PA et al: "Deletion of Phenylalanine 508 in the First Nucleotide-binding Domain of the Cystic Fibrosis Transmembrane Conductance Regulator Increases Conformational Exchange and Inhibits Dimerization."
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Sentence
Comment
220
c, overlay of spectra of Y577E NBD1 èc;RIèc;RE recorded at 1.9 mM (black) and 0.1 mM (red).
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ABCC7 p.Tyr577Glu 26149808:220:25
status: NEW236 Interestingly, a Y577E mutant did not result in significant broadening of Cys-491 and Trp-496 resonances relative to WT or to significant concentration-dependent chemical shift perturbations (Fig. 6c).
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ABCC7 p.Tyr577Glu 26149808:236:17
status: NEW238 Visual inspection indicates changes in linewidth and relative peak intensity throughout the Y577E spectra at 0.1 and 1.9 mM, strongly hinting that significant dimerization is occurring in this mutant.
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ABCC7 p.Tyr577Glu 26149808:238:92
status: NEW268 Mutations at the dimer interface (Y577K and Y577E) also affect the Q-loop segment conformational sampling.
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ABCC7 p.Tyr577Glu 26149808:268:44
status: NEW373 Furthermore, mutations in the dimer interface (Y577K and Y577E) clearly alter the Q-loop segment equilibrium, with changes observed as far away from the dimer interface as Gly-509, which is adjacent to the Phe-508 position.
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ABCC7 p.Tyr577Glu 26149808:373:57
status: NEW