ABCC2 p.Arg905Cys
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PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
227
To test for the effects of cross-linking on activity, isolated Cys-less P-gp and mutants I160C/F904C and F163/R905C were treated without or with copper phenanthroline for 10 min a 20 &#b0;C.
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ABCC2 p.Arg905Cys 25987565:227:70
status: NEW240 Cross-linking of mutants I160C/ F904C and F163C/R905C with oxidant however, inhibited more than 90% of the activity.
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ABCC2 p.Arg905Cys 25987565:240:31
status: NEW248 A study of Q loop mutations Q475A(NBD1) and Q1118A- (NBD2) suggested that the P-gp transport mechanism shows redundancy (47).
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ABCC2 p.Arg905Cys 25987565:248:27
status: NEW93 Disulfide Cross-linking Analysis-Cys-less P-gp or the ICL1/ ICL4 double cysteine mutants I160C/F904C or F163C/R905C were transiently expressed in HEK 293 cells at reduced temperature (30 &#b0;C) to promote maturation.
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ABCC2 p.Arg905Cys 25987565:93:110
status: NEW224 Mutants I160C/F904C and F163/R905C (Fig. 5, A and B) were selected to test the effects of IH1/IH4 cross-linking as the mutants yielded mature P-gp that showed efficient cross-linking in the presence of oxidant (copper phenanthroline) (Fig. 5C).
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ABCC2 p.Arg905Cys 25987565:224:29
status: NEW228 To test for the effects of cross-linking on activity, isolated Cys-less P-gp and mutants I160C/F904C and F163/R905C were treated without or with copper phenanthroline for 10 min a 20 &#b0;C.
X
ABCC2 p.Arg905Cys 25987565:228:110
status: NEW241 Cross-linking of mutants I160C/ F904C and F163C/R905C with oxidant however, inhibited more than 90% of the activity.
X
ABCC2 p.Arg905Cys 25987565:241:48
status: NEW